If each individual has such a small amount of DNA in their cells, how do the bands on the gel contain enough DNA to be visible? The Gel has to be soaked in a dye (ethidium bromide) to bind with the DNA and rinsed off after. Ethidium bromide helps to make DNA visible by glowing brightly in UV rays.
Q. What were the sizes of the DNA fragments for alleged father 1?
What were the sizes of the DNA fragments for alleged father #1? 40 mm at 700 bp and 43 mm at 600 bpData Analysis4. Which size DNA fragment did the child inherit from her mother?
Q. Which lane of the gel 2 3 4 or 5 has a DNA fragment that is about 700 base pairs?
3. Which lane of the gel, 2, 3, 4, or 5, has a DNA fragmentthat is about 700 base pairs? Fragment C contains about 700 base pairs meaning lane 4, meaning lane 4 contains a fragment that has about 700 base pairs.
Q. How do you Visualise DNA bands in gel electrophoresis?
Ethidium bromide is likely the most well-known dye used for visualizing DNA. It can be used in the gel mixture, the electrophoresis buffer, or to stain the gel after it is run. Molecules of the dye adhere to DNA strands and fluoresce under UV light, showing you exactly where the bands are within the gel.
Q. Which direction does the DNA move on the gel Why is that?
DNA fragments are negatively charged, so they move towards the positive electrode. Because all DNA fragments have the same amount of charge per mass, small fragments move through the gel faster than large ones.
Q. Does bromophenol blue glow under UV light?
Bromophenol blue is also used as a dye. At neutral pH, the dye absorbs red light most strongly and transmits blue light. (Its peak absorbance is 600nm at a basic pH of 12.) At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution.
Q. Why is bromophenol blue used?
Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis.
Q. Why is bromophenol blue yellow?
Bromophenol Blue is a pH indicator dye that turns yellow under acidic conditions. Bromophenol Blue has a pI below pH 4.0. The concentrated dye solution may have a reddish or greenish cast to the color. If the pH gradient of the gel includes the pI of the dye, it will turn yellow at its pI.
Q. Why is bromophenol blue a good indicator?
It changes colour from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. Bromophenol blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis (2), and also used as a dye. Bluish-violet coloured solution.
Q. Is bromophenol blue a chromophore?
This compound, shown in Fig. 1, has a characteristic of 589 nm. Bromophenol Blue belongs to a j max group of related compounds that have the same phenol- phthalein chromophore, Fig. 2.
Q. Is bromophenol blue a weak acid?
Explanation: The idea here is that bromophenol blue acts as a weak acid in aqueous solution. As you can see, this equilibrium is influenced by the pH of the solution, i.e. by the concentration of hydronium cations present in the solution.
Q. Does bromophenol blue stain bacteria?
Bacterial isolates G and H were found capable of degrading both Crystal violet and Bromophenol blue dyes to a greater extent within 120 hours of incubation. The present investigation revealed these isolates can be used for treatment of effluents contaminated with Crystal violet and Bromophenol blue.
Q. What is the best indicator for HCl and NaOH?
Methyl orange
Q. What is the color of bromophenol blue in an acid?
| Indicator | pH Range | Acid |
|---|---|---|
| 6.0 Bromocresol purple | 5.2-6.8 | yellow |
| 7.0 Bromophenol blue | 6.0-7.6 | yellow |
| 9.0 Chlorophenol red | 4.8-6.7 | yellow |
| 11 Congo red | 3.0-5.0 | yellow |
Q. Which indicator is not used for weak base and strong acid titration?
(ii) Weak acid Vs strong base: Phenolphthalein. (iii) Strong acid Vs weak base: Methyl red and methyl orange. (iv) Weak acid vs. weak base: No suitable indicator can be used for such a titration.
Q. Which indicator is best for weak base and strong acid?
methyl orange
Q. Is NH3 a weak acid or base?
NH3, or ammonia, is typically a weak base.
Q. Which is the best choice for weak base-strong acid titration?
methyl red
Q. What happens when you mix a strong acid and a weak base?
As a general concept, if a strong acid is mixed with a weak base, the resulting solution will be slightly acidic. If a strong base is mixed with a weak acid, the solution will be slightly basic.
Q. What is strong acid weak base titration?
In a weak base-strong acid titration, the acid and base will react to form an acidic solution. This results in a solution with a pH lower than 7. An example of this is the titration of hydrochloric acid (strong acid) into ammonia (weak base), which forms the conjugate acid ammonium and produces an acidic solution.
Q. What indicator is a strong acid weak base?
Choosing an appropriate indicator for a titration
| pH | 0 | Use |
|---|---|---|
| thymol blue (1st change) | < 1.2 | strong acid + weak base |
| bromophenol blue | < 3.0 | strong acid + weak base |
| methyl orange | < 3.1 | strong acid + weak base |
| bromocresol green | < 3.8 | strong acid + weak base |
Q. What is strong acid and weak base?
Strong acids and bases are 100% ionized in aqueous solution. Weak acids and bases are less than 100% ionized in aqueous solution. Salts of weak acids or bases can affect the acidity or basicity of their aqueous solutions.
Q. What are the 7 weak bases?
Examples of Weak Bases
- Ammonia (NH3) Ammonia is a chemical compound with the formula NH3.
- Trimethylamine (N(CH3)3) Trimethylamine, often abbreviated to TMA, is a weak base with the chemical formula N(CH3)3.
- Pyridine (C5H5N) Pyridine is an organic compound with the chemical formula C5H5N.
