Colistin methanesulfonate was used in all patients. The daily dosage of nebulized colistin was 150 mg divided into 2 doses, and 75 mg of colistin was diluted in 4 mL of sterile normal saline. The solution was nebulized through a conventional nebulizer.

Add the 3 mL of 0.9% sodium chloride or sterile water to the Colistimethate vial. Remove the syringe needle and recap the needle. Gently swirl or roll the vial of Colistimethate in your hands until the powder has dissolved. Vigorous shaking will create a lot of foam.

Aerosolized colistin may be an effective adjunctive intervention for the treatment of nosocomial pneumonia due to multidrug-resistant Gram-negative bacteria in patients without cystic fibrosis.

Nebulization of high-dose colistin was effective to treat VAP caused by multidrug-resistant P. aeruginosa or A. baumannii. Its therapeutic effect was noninferior to intravenous β-lactams associated with aminoglycosides or quinolones for treating VAP caused by susceptible P. aeruginosa and A.

Colistin binds to LPSs and phospholipids in the outer cell membrane of Gram-negative bacteria. It competitively displaces divalent cations (Ca2+ and Mg2+) from the phosphate groups of membrane lipids, which leads to disruption of the outer cell membrane, leakage of intracellular contents and bacterial death (Figure 3).

Insert the syringe into the hole at the top of the vial and draw up the prescribed amount 0.9% sodium chloride. sodium chloride into the vial of Colomycin. Put the rubber bung back on and roll the Colomycin carefully to mix the dry powder with the 0.9% Sodium Chloride. antibiotic solution into the nebuliser chamber.

Nebulisers are used to turn medicines into a fine mist, which can be inhaled into the lungs. Colomycin (colistimethate sodium, also sometimes known as colistin) is an antibiotic which can be used to treat long-term respiratory infections, especially those caused by a bacterium called Pseudomonas aeruginosa.

For bolus injection: Reconstitute the contents of the vial with not more than 10ml water for injection or 0.9% sodium chloride. For infusion: The contents of the reconstituted vial may be diluted, usually with 50ml 0.9% sodium chloride.