Inoculum was prepared by transferring cells of S. cerevisiae into a 500 mL flask containing 100 mL of the culture medium (20 g/L glucose, 3 g/L peptone, 4 g/L yeast extract; pH 7.0), and incubating this at 30°C for 12 h.

The fermentation process involves an inoculum development step, which is the preparation of a population of microorganisms from a stock dormant culture to a state useful for inoculating a final production fermenter.

The process is a stepwise and gradual increase in scaling-up the volume of inoculum to the desired level, which includes preparation of bacterial suspension (either vegetative cells or spores) in sterile tap water and then to the broth or in case of fungi, their hyphae are transferred to the broth. …

Inoculation is the process of adding yeast to juice/must to begin fermentation. This is typically done immediately following solids separation for white grape varieties, and after some period of cold soaking for red varieties.

Definition. noun, plural. (1) Cells used in an inoculation, such as cells added to start a culture. (2) A biological material (like a virus or toxin or immune serum) that is injected into a human to induce or increase immunity to a particular disease.

Inoculum preparation involves obtaining the organisms in an optimal state that is compatible with inoculation into cell culture, tissue culture, media, and fermentors. The prime objective is usually to achieve a high level of viable biomass in a suitable physiological state for use as an inoculum.

The inoculum source had a significant impact on both growth and pH tolerance of the bacterial community in the EL soil. This study showed the connection between the development of function (growth), community properties (pH tolerance) and the structure of the bacterial community.

Inocula are prepared by growing C. neoformans in liquid YPAD overnight at 30 °C. Cells are counted by hemocytometer and, for an intranasal infection, 1×107 cells are washed twice with PBS and resuspended in 1 ml of PBS.

The inoculum source had a significant impact on both growth and pH tolerance of the bacterial community in the EL soil. These differences between the EL soil inoculated with UL soil and FL soil were correlated to structural changes, as evidenced by both PLFA and DGGE analyses on the soil.

During each stage of the inoculum development, aseptic conditions are maintained, and the pH, temperature, and carbon and nitrogen concentrations are controlled strictly. The fermentation is carried out over 72–96 h, and the viscosity and biomass are monitored constantly.

Temperature has influence on fermentation – to some extent temperature increases the yeast growth, speed of enzyme action (approximately doubles with every 10 °C rise), cell sensitivity to the toxic effect of alcohol increases with temperature due to increased membrane fluidity.

The definition of an inoculum is a substance introduced into the body to create or increase the body’s resistance or immunity to a disease. A flu vaccine is an example of an inoculum. The active material used in an inoculation; an inoculant.

Development of active logarithmic microbial culture that is suitable for the final industrial production level is known as inoculum development. Inoculum we use for industrial fermentations should be • In its active, healthy and exponential growth phase.

Development of active logarithmic microbial culture that is suitable for the final industrial production level is known as inoculum development. Inoculum we use for industrial fermentations should be • In its active, healthy and exponential growth phase. Available free of contamination in required large volumes.

Inoculum development for vegetative fungi. This was achieved by using a four-stage inoculum development programme (initiated by a spore-inoculated shake flask) with very rich media in the third and fourth cultures. Thus, this system required a dispersed vegetative inoculum to generate a pelleted production fermentation.

To prepare growth medium, the mineral medium is supplemented with a defined amount of pure glycerol. Inoculum Development: Seed cultures were repeatedly subcultured in flasks with 5% v/v inoculum size.