approximately 14 SqM

To get a standard 100uM concentration, you must add the nmol*10 volumen (uL). For instance, if your oligo was synthesized and the nmol yield is 44.2, then you must add 442uL of nuclease-free water to get 100 uM concentration.

You will need one gallon of primer or paint to cover 250 square feet of wall. This estimate works best for acrylic primers and paints, and takes into account different application methods and rougher surface textures. Generally, the rougher a surface is the more primer or paint it will take.

The primer coat doesn’t have to be perfect, but it should cover the surface (no bare spots) and it shouldn’t be so blotchy that you get drips or visible unevenness. You need to prime the surface thoroughly. The purpose of the primer is so give the surface uniform absorbtion properties.

You will want to use 2-3 coats of primer to ensure there is a good bond between the new paint and the wall, and also to cover up any previous colors, especially if they are red, orange, or a strange outdated color. In short, you will typically need 2 coats of primer for most painting projects.

A normal color requires two coats of primer. If you have to think about it a little more, it probably takes three coats of primer. Depending on the color you choose next–if it is a medium shade or dark–one layer less of primer could be good enough.

It’s a high quality, interior or exterior paint that has increased adhesion and stain blocking capability. Most high-quality interior paints when applied directly to a bare or previously painted surface will stick, cover and look similar once finished. A paint and primer in one is not designed for every project.

In most cases, latex primers don’t take more than an hour to dry out. However, you should wait three to four hours before applying a layer of paint. On the other hand, an oil-based primer will need a longer time to dry out. You should give it 24 hours to make sure that it’s completely ready for another coat.

One coat of primer is usually all it takes. You may need two coats, however, if you are priming over knots or stains and they are still visible after the first coat dries. You can usually skip the primer if you’re painting over old paint and you’re not dramatically changing the color.

Easy Ways to Make Interior Paint Dry Faster

1. Apply Light Coats. While it might be tempting to try to get as much paint as you can on the wall at once, using thinner coats will help your paint dry faster.
2. Paint One Wall at a Time. If you focus on painting one wall at a time, then you give the other walls time to dry.
3. Use a Hair Dryer or Heater.
4. Keep the Air Moving.

Applying the second coat too early will result in streaks, peeling paint, and uneven color. Not only will this ruin the entire project but it’ll cost additional money to get more paint in some occasions. It’s best to wait for the first coat to dry.

Oil-based paint may feel dry two to four hours after it’s applied. But recoat a full 24 hours after you’ve painted to make sure the surface is completely dry and ready for another round of paint.

Paint that isn’t allowed to dry before the application of a second coat is likely to peel, streak, clump, or flake when dry. Experts recommend leaving a minimum of two to four hours of drying time between each coat for best results.

Depending on the color and condition of the surface to be coated, sometimes one coat of paint is just not possible. One coat of paint does not offer full color coverage, so the base color often bleeds through and alters your true color choice. Durability is also better with two coats of paint.

If the new color is “close” to the existing color, then generally only one coat is needed, even in the case of darker colors. Creating a substantial color change, or painting over white usually requires a second coat to prevent the underlying color from “peeking through”.