Once you have the highest magnification lens in place, look into the eyepiece. It is very important that you move very slowly, stopping to check how close the lens is moving to the slide. At this point, if you use the coarse adjustment knob you will break the slide and scratch the lens.

Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. Using the coarse focus with higher lenses may result in crashing the lens into the slide.

Use the focus knob (4) to place the sample into focus and readjust the condenser (7) and light intensity for the clearest image (with low power objectives you might need to reduce the light intensity or shut the condenser).

Avoid touching the glass of the bulb with your bare hands, as fingerprints and particles of dust left on the bulb can burn into the bulb and reduce the life of the bulb as well as the quality of the light produced by the bulb.

Coarse focus is used to find subject matter and fine focus is used to zoom in to view inside the subject matter. What part of the microscope must be used when changing from one objective lens to another?

Answer: Compound microscopes can magnify specimens enough so that the user can see cells, bacteria, algae, and protozoa. You cannot see viruses, molecules, or atoms using a compound microscope because they are too small; an electron microscope is necessary to image such things.

Use the coarse focus to raise the stage all the way up until it blocks. The objective at the highest position, the 4x objective should of course not touch the slide. Close the condenser and looking through the eyepiece lower the stage using the coarse focus until you see a clear image.

Fine and coarse adjustment controls: Adjust the focus of the microscope by moving the stage vertically. The coarse adjustment knob is moved to its highest position stop (forward rotation). The fine adjustment knob is used to bring the image into sharp focus.

Use ONLY the fine focus control when focusing the higher power objectives (20X, 40X, 100X) on a slide. The course focus control is too course for focusing with these objectives. Objectives are fragile and must not be rammed into slides.

Fine Focus: A knob used to fine-tune the focus of a specimen in conjunction with the coarse focus. Field of View: The diameter of the circle of light seen through a microscope. Focus: The ability to achieve a clear image, typically achieved by moving either the eyepiece tubes or the stage.

Fine Adjustment knob. part of the microscope that is used for focusing finer details of specimen being viewed. Objectives like low power and high power objectives are used with fine Adjustment knob for clearer image in higher resolution.

Answer: Eyepiece Lens: The lens at the top of the microscope that you look into. Field of View: Sometimes abbreviated “FOV”, it is the diameter of the circle of light that you see when looking into a microscope. As the power gets greater, the field of view gets smaller.

A light microscope is a biology laboratory instrument or tool, that uses visible light to detect and magnify very small objects, and enlarging them. They use lenses to focus light on the specimen, magnifying it thus producing an image. The specimen is normally placed close to the microscopic lens.

A compound microscope uses two or more lenses to produce a magnified image of an object, known as a specimen, placed on a slide (a piece of glass) at the base. The microscope rests securely on a stand on a table. The specimen has to be a very thin slice so light rays will pass through.