Electron beams are dangerous and can only be used sparingly. You cannot view live specimens because the necessary preparation kills cells. Resolution is weak and cannot make out details of very small specimens.
Q. Which kind of lenses are in a light microscope?
Principles. The light microscope is an instrument for visualizing fine detail of an object. It does this by creating a magnified image through the use of a series of glass lenses, which first focus a beam of light onto or through an object, and convex objective lenses to enlarge the image formed.
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Q. What are the limitations of a light microscope?
List of the Disadvantages of Light Microscopes
- Light microscopes do not magnify at the same level as other options.
- Light microscopes have a lower resolution.
- Light microscopes make it challenging to view living internal structures.
- Light microscopes cannot operate in darkness.
Q. What are the pros and cons of using electron and compound microscopes?
pros and cons
| compound light microscope | |
|---|---|
| + | easy to use |
| + | inexpensive related to electron microscopes |
| + | can look at live samples |
| – | Viruses, molecules and atoms cannot be viewed (viewed only with an electron microscope.) |
Q. Is there any disadvantage of using a compound microscope?
A major problem when observing under compound microscope is that the image doesn’t have a big contrast. One other problem for compound microscope is that it can only magnify till certain extent. Anything smaller then that limit then we can’t observe.
Q. What are the advantages of the two main types of microscope?
With two lenses, the compound microscope offers better magnification than a simple microscope; the second lens magnifies the image of the first. Compound microscopes are bright field microscopes, meaning that the specimen is lit from underneath, and they can be binocular or monocular.
