An inverted microscope is a microscope with its light source and condenser on the top, above the stage pointing down, while the objectives and turret are below the stage pointing up. It was invented in 1850 by J. Lawrence Smith, a faculty member of Tulane University (then named the Medical College of Louisiana).

Inverted microscopes are significantly more expensive than conventional instruments. Inverted-microscope prices range from approximately $1,000 to $10,000, with higher-priced instruments coming attached to a camera and/or being capable of phase contrast and fluorescence microscopy.

An inverted microscope gives you greater freedom than an upright one. Inverted microscopes enable you to look at more samples in a shorter period of time. With an inverted microscope, you cannot crash an objective into the sample. Inverted microscopes save you time and money in sample preparation.

Inverted Microscope Inverted microscopes are popular for live cell imaging, because: Cells sink to the bottom and onto the coverslip for adherence. Sample access from the top (e.g., for liquid exchange or micropipettes) No contact between objective and sample—sterile working conditions are possible.

Most of the fluorescence microscopes used in biology today are epi-fluorescence microscopes, meaning that both the excitation and the observation of the fluorescence occur above the sample. Most use a Xenon or Mercury arc-discharge lamp for the more intense light source.

Moving it clockwise will lower it; counter- clockwise will raise it. In most cases it can be left all the way up, though it may need adjustment when you use a 100x objective lens to achieve a clear image. The iris diaphragm replaces the disc diaphragm on the 4100SPL and 4100DXL models.

The first disadvantage is cost. Inverted microscopes are not anywhere near as common as a microscope with a standard configuration so there is less competition both in the new and used markets. Further, they are more complex and therefore expensive to build.

The two basic types of inverted microscopes include biological inverted microscopes and metallurgical inverted microscopes.

A fluorescence microscope can cost between $2,400 and $21,000+ depending on the specifications and customizations that you require.

The conventional microscope uses visible light (400-700 nanometers) to illuminate and produce a magnified image of a sample. A fluorescence microscope, on the other hand, uses a much higher intensity light source which excites a fluorescent species in a sample of interest.

Place ZEISS Primovert right inside your Laminar Flow Box. Examine unstained cells in phase contrast and GFP-labeled cells in fluorescence contrast quickly and efficiently. The inverted microscope is especially perfect for cancer and genetic research.

Biomedical research on living cells was revolutionized in 1934 when the Dutch physicist Frits Zernike described the concept of phase contrast. Within two years ZEISS was applying Zernike’s original design in the first prototype phase contrast microscopes.

Widefield microscopes are available in different versions. Choose an upright microscope if your task is to analyze zebrafish embryos, stained tissue selections or brain slices. For tissue culture and quick assessments in routine research, the inverted microscope might be the better choice.

Six different stand versions for superior performance, unsurpassed ZEISS optics and maximum ease of use. Sophisticated operating concept and open interfaces for external communication and network connectivity – future proof. Easy to use microscope – perform complex tasks reliably, produce results of impressive quality.