The NAC (NAM, ATAF and CUC) family is one of the largest plant-specific transcription factor (TF) families. They affect plant immunity through their regulatory impact on signaling of plant hormones, which in turn are key players in plant immune responses.

Transcription factors are proteins involved in the process of converting, or transcribing, DNA into RNA. Other transcription factors bind to regulatory sequences, such as enhancer sequences, and can either stimulate or repress transcription of the related gene.

We described the high-throughput identification of DNA-binding specificities of 63 plant transcription factors (TFs) and their relevance as cis-regulatory elements in vivo.

Chromatin immunoprecipitation, or ChIP, is an antibody-based technology used to selectively enrich specific DNA-binding proteins along with their DNA targets. ChIP is used to investigate a particular protein-DNA interaction, several protein-DNA interactions, or interactions across the whole genome or a subset of genes.

Chromatin immunoprecipitation (ChIP) assays identify links between the genome and the proteome by monitoring transcription regulation through histone modification (epigenetics) or transcription factor–DNA binding interactions.

Background. Posttranslational modification of histone tails such as histone 3 lysine 27 acetylation (H3K27ac) is tightly coupled to epigenetic regulation of gene expression.

Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell. The DNA-protein complexes (chromatin-protein) are then sheared into ~500 bp DNA fragments by sonication or nuclease digestion.

Insurance program that provides low-cost health coverage to children in families that earn too much money to qualify for Medicaid but not enough to buy private insurance. In some states, CHIP covers pregnant women.

It is a mark that indicates acetylation of the lysine residue at N-terminal position 27 of the histone H3 protein. H3K27ac is associated with the higher activation of transcription and therefore defined as an active enhancer mark. H3K27ac is found at both proximal and distal regions of transcription start site (TSS).

Cruciferous vegetables Aside from being detoxification superfoods, crucifers are also considered methylation adaptogens thanks to their sulforaphane and folate content. Cruciferous vegetables include arugula, broccoli, cabbage, Brussels sprouts, cauliflower, kale, and radish.

DNA demethylation is the process of removal of a methyl group from cytosines. DNA demethylation can be passive or active. The passive process takes place in the absence of methylation of newly synthesized DNA strands by DNMT1 during several replication rounds – for example, upon 5-azacytidine treatment [23].

Active DNA demethylation

Evidence suggests that DNA methylation of the gene body is associated with a higher level of gene expression in dividing cells (Hellman and Chess, 2007; Ball et al, 2009; Aran et al, 2011).