INTENDED USE. Remel TB Decolorizer is a reagent recommended for use with the Kinyoun or Ziehl-Neelson carbolfuchsin staining procedure to differentiate acid-fast bacteria from nonacid-fast bacteria.

The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat.

Acidic Dyes: It is dye which has negative charge so they bind to positively charged cell structures like some proteins. Since, surface of bacterial cells are negatively charged(due to Teichoic acid), basic dyes are most commonly used in bacteriology. Examples: Crystal Violet, Methylene Blue, Safranin , basic fuschin.

A counterstain, such as the weakly water soluble safranin, is added to the sample, staining it red. Since the safranin is lighter than crystal violet, it does not disrupt the purple coloration in Gram positive cells.

Introduction. Safranin is a cationic dye used in histology and cytology to distinguish and identify different tissues and cells. It is popular in medical research for staining acidic proteoglycan that is found in cartilage tissues, enabling researchers to analyze cell chondrogenesis.

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Acid-fast bacteria are red; non-acid-fast cells are blue. Uses heat to stain endospores with malachite green (Schaeffer-Fulton procedure), then cell is washed and counterstained with safranin.

Acid-fast bacteria, also known as acid-fast bacilli or simply AFB, is a group of bacteria sharing the characteristic of acid fastness….These include:

• Bacterial endospores.
• Head of sperm.
• Cryptosporidium parvum.
• Isospora belli.
• Cyclospora cayetanensis.
• Taenia saginata eggs.
• Hydatid cysts.
• Sarcocystis.

Acid Fast Bacteria: Mycobacterium is an example of acid fast bacteria. Non Acid Fast Bacteria: Escherichia coli is an example of acid fast bacteria.

A normal result for an acid-fast bacteria smear is negative, meaning no bacteria were found in the sputum sample. A positive result means that bacteria were found and that you may have an infection. The smear is treated with a special acid-fast stain that can provide a preliminary test result in 24 hours.

A positive test result from the acid-fast stain confirms the patient has TB. In other types of acid-fast bacteria such as Nocardia, only certain parts of each cell retain the dye, such as the wall of the cell. A positive test result from a partial or modified acid-fast stain identifies these types of infections.

Acid alcohol is a differentiation reagent. It is used in various staining methods, most frequently in regressive hematoxylin eosin (HE) staining and provides excellent differentiation between nuclear and non-nuclear structures. Differentiation rinses dyes from cytoplasm while the nucleus remains stained.

Acid alcohol: Mix 2.0 mL concentrated hydrochloric acid and 98.0 mL 95% ethyl alcohol. 3. Methylene blue: Prepare a saturated solution of methylene blue by adding 1.5 g powdered methy- lene blue to 100 mL 95% ethyl alcohol. Slowly add the alcohol to dissolve the powder.

Hydrochloric acid is the water-based, or aqueous, solution of hydrogen chloride gas. It is also the main component of gastric acid, an acid produced naturally in the human stomach to help digest food.

What is the function of the counterstain in the acid-fast staining procedure? The counterstain stains non-acid-fast bacteria blue if using Methylene Blue or green if using Brilliant Green.

The dye or stain that is used to differentiate one component or cellular structure from another, or to differentiate an entity from another in a specimen.

The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.

The negative stain is particularly useful for determining cell size and arrangement. It can also be used to stain cells that are too delicate to be heat-fixed. We use nigrosin as our negative stain. Nigrosin is an acidic stain.

The advantages of the negative stain include the use of only one stain and the absence of heat fixation of the sample. Negative staining employs the use of an acidic stain and, due to repulsion between the negative charges of the stain and the bacterial surface, the dye will not penetrate the cell.

Some suitable negative stains include ammonium molybdate, uranyl acetate, uranyl formate, phosphotungstic acid, osmium tetroxide, osmium ferricyanide and auroglucothionate. The structures which can be negatively stained are much smaller than those studied with the light microscope.

Negative staining is used when it is important to be able to view the bacteria without using harsh stains or performing the heat fixing technique that could possibly distort or change the shape of the bacteria. It is used when looking at capsules and yeast or spirochetes that do not stain well.