Fluoro-Gel is a water based mounting medium designed for the permanent mounting of fluorescent stained tissues, which may either be damaged or soluble in organic solvents, such as xylene or toluene.

Anti-Fade Fluorescence Mounting Medium ab104135 (previously called Fluoroshield Mounting Medium) is an aqueous mounting medium designed to preserve fluorescence when imaging tissues and cell samples. Fluorescence is retained during prolonged storage at 4°C in the dark.

Mounting medium is the medium that your sample is in while it is being imaged on the microscope. The simplest type of mounting medium is air, or a saline-based buffered solution, such as PBS.

Filters. Referring to something that resists the dimming (fading) of color. The chemical additives in the anti-fade paint meant the color stayed vibrant year after year.

Propylene glycol is the dye solvent so the dye solvent will not dissolve out any of the tissue lipid. Aqueous mounting media, such as glycerin jelly is used to mount the finished slide because the organic solvents present in synthetic resins would dissolve the dye-lipid complex.

At their most basic, mounting media adhere your specimen to a glass slide and coverslip and in doing so, provide a protective benefit to your sample. Some mounting media have been developed with additional benefits including: To prevent cells from drying out. To prevent signals from fading or photobleaching.

To preserve a section of colored tissue for optical microscopy, it is necessary to put it on a glass slide and cover it with a thin glass slice (coverslip). A mounting medium is used to adhere the coverslip to the slide and to preserve the marked sample during handling and storage. …

The mounting medium is the solution in which the specimen is embedded, generally under a cover glass. Simple liquids like water or glycerol can be considered mounting media, though the term generally refers to compounds that harden into a permanent mount.

The main purpose of mounting media is to physically protect the specimen; the mounting medium bonds specimen, slide and coverslip together with a clear durable film. The medium is important for the image formation as it affects the specimen’s rendition….

In our experience n-propyl gallate added to mounting media reduces fading of fluorescence from many different fluorophores during fluorescence microscopy. The following is a simple recipe for making an anti-fade mounting medium containing n-propyl gallate.

Mount samples in fluorescence antifade mounting media such as EverBrite™ Mounting Medium (medium with DAPI can be used for blue nuclear counterstaining). For chambered coverglass or multi-well coverglass plates, remove all traces of buffer and add enough mounting medium to completely cover the cells.

For fluorescent cell and tissue staining, Abcam recommends this product (Fluorescence Mounting Medium ab104135), Mounting Medium with DAPI ab104139 , and Mounting Medium with PI ab104129. For thick sections or tissues containing lots of fat, we recommend Glycerol Mounting Medium with DAPI ab188804.

Protocol: Immunofluorescence Staining of Cells for Microscopy. Use the same volume for washes as you would for cell culture medium (we use 100 uL per well of a 96-well plate). For some cell types, buffer with Ca 2+ /Mg 2+ may be necessary to prevent cell rounding and detachment.