Plants important to developing countries that have been grown in tissue culture are oil palm, plantain, pine, banana, date, eggplant, jojoba, pineapple, rubber tree, cassava, yam, sweet potato, and tomato.

Types of Plant tissue culture

• Seed Culture.
• Embryo Culture.
• Callus Culture.
• Organ Culture.
• Protoplast Culture.
• Anther Culture.

Some plant enthusiasts and hobbyists view R. tetraspermas grown via tissue culture as unhealthy and undesirable. Tissue culture works by choosing a healthy, quality mother plant to clone, but the plantlets propagated from the mother plant are rarely grown in the exact same conditions as the mother plant.

Liners should be grown in containers for several months before being planted into the ground. Use a well drained, bark based, soilless mix. A constant liquid feed of 20-10-20 at 50ppm nitrogen is recommended. Maintain a soil PH of 6.0-6.5.

The tissue culture process allows you to get more cells, new cells, or tissue, from existing plant matter. When the process is done in a lab, expensive equipment is used, however, when performed at home, relatively common household items can be used for DIY Tissue Culture.

In 1907, Ross Granville Harrison, an American zoologist, was able to culture the nerve cells from a frog in solidified lymph. Because of his contributions to the tissue culture method, Harrison now has the title of Father.

Tissue culture is a technique in which fragments of plants are cultured and grown in a laboratory. The media used for the growth of the culture is broth and agar. This technique is also known as micropropagation.

The shelf life on these things are pretty long. I had some tissue cultured Dwarf Hairgrass and it lasted easily 3 weeks sealed under lighting.

Advantages of Tissue Culture The new plantlets can be grown in a short amount of time. Only a small amount of initial plant tissue is required. The new plantlets and plants are more likely to be free of viruses and diseases. The process is not dependant on the seasons and can be done throughout the year.

Blue hosta varieties aren’t technically blue. The plants’ leaves are covered in a wax-like coating, which makes them appear to have that blueish hue. This is also described as “glaucous,” which is Latin for greyish blue and used to describe plants with those tones or others that are blue-green.

Join the on line Freecycle.org in your area, many offer plants that are being thinned out. You can also make a request for Hosta plants.

Disadvantages of Tissue Culture

• Tissue Culture can require more labor and cost more money.
• There is a chance that the propagated plants will be less resilient to diseases due to the type of environment they are grown in.

Tissue culture can produce higher amounts of better quality propagated hostas but the process is costly. Another major negative to tissue culture is the risk of bacterial contamination which can greatly reduce the number of successful propagation.

Tissue culture is used on many herbaceous plants in the ornamental industry especially hostas. Tissue culturing hostas allows for a large quantity of hosta to be produced with the need of few donor plants.

A small hosta plant will start to grow and this is calle d the plantlet. It has vegetative tissue and roots growing. The plantlets are removed from the gel and the roots are washed to remove the gel media. The plantlet is than transplanted into potting soil inside a greenhouse.

Hostas are a very desirable herbaceous perennial due to their shade tolerance and attractive foliage. The are in the genus of hosta and in the Asparagaceae family. Hosta’s are native to China, Japan and Korea and were introduced into the US in the mid-1800s.