Q. Why do we incubate the inoculated agar plates?
Petri dishes need to be incubated upside-down to lessen contamination risks from airborne particles landing on them and to prevent the accumulation of water condensation that could disturb or compromise a culture.
Q. What needs to be done when there is spillage of the inoculum?
Wearing disposable gloves, disinfect the area by covering the spill with several layers of paper towel/cloth soaked in a suitable disinfectant (see Commonly available disinfectants and their uses, page 7) and leave for 15–30 minutes. Spill debris should then be swept into a dustpan using paper towels.
Q. What are the important techniques in performing bacterial inoculation process?
The USP describes two general methods for conducting the test: the direct transfer, or direct inoculation, method and the membrane filtration method. As the name indicates, the direct inoculation method involves the aseptic transfer of a sample of test product solution into the sterility test growth medium.
Q. When a culture media is inoculated Why is the loop sterilized after the initial inoculum is put on?
7 Cards in this Set
| When an agar plate is inoculated why is the loop sterilized after the initial inoculum is put on? | So you don’t cross contaminate |
|---|---|
| Pure culture definition | Contains one species |
| Mixed culture definition | Contains more than one species |
Q. What does Defibrinated blood mean?
Whole blood from which fibrin has been removed. It does not clot.
Q. How does Streptococcus affect the body?
(Strep Infections) These infections affect various areas of the body, including the throat, middle ear, sinuses, lungs, skin, tissue under the skin, heart valves, and bloodstream. Symptoms may include red and painful swollen tissues, scabby sores, sore (strep) throat, and a rash, depending on the area affected.
Q. What happens if strep gets into your bloodstream?
Blood infections: The strep bacteria can also get into your bloodstream, where they don’t normally live. This is called “bacteremia.” If the strep bacteria release toxins in multiple organs, it can create another rare, life-threatening condition called “streptococcal toxic shock syndrome” that can cause organ failure.
Q. Can Streptococcus be cured?
Strep throat is an infection in the throat and tonsils caused by a bacterium known as Streptococcus. With proper treatment, strep is usually cured within 10 days. Treatment includes antibiotics such as penicillin or amoxicillin.
