Why is it necessary to use oil when viewing a specimen with the oil immersion lens? Check all that apply. – The oil reduces the diffraction of light. The resolving power of the human eye is higher than that of the light microscope whereas the magnification of the light microscope is higher than that of the human eye.

Immersion oil is placed between slide and oil immersion lens to preserve the direction of light rays at the highest magnification. Immersion oil has same refractive index as glass. Oil has same effect as increasing the objective lens diameter – improving resolving power of the lense.

By placing immersion oil between the glass slide and the oil immersion lens (100X), the light rays at the highest magnification can be retained. Immersion oil has the same refractive index as glass so the oil becomes part of the optics of the microscope.

In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.

Immersion Oil contributes to two characteristics of the image viewed through the microscope: finer resolution and brightness. These characteristics are most critical under high magnification; so it is only the higher power, short focus, objectives that are usually designed for oil immersion.

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Key takeaways. The microscope immersion oil decreases the light refraction, allowing more light to pass through your specimen to the objectives lens. Therefore, the microscope immersion oil increases the resolution and improve the image quality.

Oil Immersion Objective Lens (100x) The oil immersion objective lens provides the most powerful magnification, with a whopping magnification total of 1000x when combined with a 10x eyepiece.

Total magnification = 10 X 10 = 100X (this means that the image being viewed will appear to be 100 times its actual size).