Gel filtration based on Sephadex enables group separation of biomolecules that are above the exclusion limit of the medium, from contaminants such as salts, dyes, and radioactive labels. Sephadex is prepared by cross-linking dextran with epichlorohydrin.

SEC based on Sephadex chromatography resins enables desalting and buffer exchange of biomolecules. Sephadex is prepared by cross-linking dextran with epichlorohydrin. The exclusion limit and the fractionation range are controlled by varying the degree of cross-linking of the resin.

Sephadex® G-50 Fine is suitable for purification of DNA from small molecules by gel filtration (exclusion limit: 20 bp dsDNA (rA)20).

Sephadex® is composed of repeating glucose units attached by α-1,6 glucosidic bonds. This structure ensures more aptamer binding sites and greater binding capacity. It is suitable for large-scale purification and is considered stable and can be utilized more than once.

Applications. Sephadex is used to separate molecules by molecular weight. Sephadex is a faster alternative to dialysis (de-salting), requiring a low dilution factor (as little as 1.4:1), with high activity recoveries.

Hydrophobic long-chain alkyl ethers of Sephadex have been synthesized and tested for use in liquid chromatography. Examples are given of straight-and reversed-phase separations of waxes, fatty acids, glycerides, glycerol ethers, sterols, bile acids, and hormonal steroids.

Sephadex is a gel filtration resin prepared by crosslinking dextran with epichlorohydrin. Sephadex G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-25 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine).

Sephadex G-50 Superfine is a well established gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine’s small bead size give higher efficiency. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Sephadex® G-50 Fine is well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Fine´s small bead size give higher efficiency. Sephadex® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin.

Sephadex G-10, G-15, G-25, G-50, G-75, and G-100 are based on dextran of different molecular weight ranges. The lower the G-value, the higher the degree of cross-linking of the dextran polymers, and the lower the molecular weight fractionation range.

Sephadex is a cross-linked dextran gel used for gel filtration. By varying the degree of cross-linking, the fractionation properties of the gel can be altered. These highly specialized gel filtration and chromatographic media are composed of macroscopic beads synthetically derived from the polysaccharide dextran.

DEAE-Sephadex is a positively-charged slurry that will have electrostatic interactions with the negatively charged atoms, making them elute later than the positively-charged molecules in the interested sample. This is a separation technique used widely to discover specific proteins, or enzymes in the body.

Application Sephadex®G-100 is a gel filtration medium used in protein chromatography, affinity chromatography and gel filtration chromatography It has been used: in gel filtration column for α-amylase purification

The lot specific COA document can be found by entering the lot number above under the “Documents” section. My Sephadex® G-100 column is cracking. What is the maximum operating pressure that Product G100120 can take? The maximum operating pressure for this gel is 96 cm water = 1.6 psi.

Sephadex G-25 column chromatography was used for the separation of bound 3 H-d-aldosterone from free 3 H-d-aldosterone. 150 mm × 9 mm columns were prepared. Tris-CaCl 2 buffer (pH 7.4) was used as eluant, except where otherwise stated, and 0.5 ml fractions of eluate were collected. Fractions were assayed for radioactivity and protein content.

Sephadex is a trademark for cross-linked dextran gel used for gel filtration. It was launched by Pharmacia in 1959, after development work by Jerker Porath and Per Flodin. The name is derived from separation Pharmacia dextran.